| 质粒名称: | pAM-PAT-ProCDKA;1 GW |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 79750 |
| 存储实验室: | Nico Dissmeyer, Arp Schnittger |
|
载体骨架基本信息 |
|
| 载体名称: | Binary vector pAMPAT-MCS |
| 完整质粒大小: | 8400 bp |
| 载体类型: | Plant Expression |
| 筛选标记: | Basta |
| 载体抗性: | Chloramphenicol and Ampicillin |
| 生长温度: | 37 ℃ |
| 宿主菌: | ccdB Survival |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 启动子: | ProCDKA;1 |
| 克隆方法: | Gateway Cloning |
| 5’测序引物 | gaccggcaacaggattcaatcttaag |
| 3’测序引物 | GAATTAGAAATTTTATTGATAGAAG |
|
文献引用方法 |
|
| 材料和方法部分: | pAM-PAT-ProCDKA;1 GW was a gift from Nico Dissmeyer & Arp Schnittger (Addgene plasmid # 79750 ; http://n2t.net/addgene:79750 ; RRID:Addgene_79750) |
| 参考文献部分: | A positive signal from the fertilization of the egg cell sets off endosperm proliferation in angiosperm embryogenesis. Nowack MK, Grini PE, Jakoby MJ, Lafos M, Koncz C, Schnittger A. Nat Genet. 2006 Jan;38(1):63-7. Epub 2005 Nov 27. 10.1038/ng1694 PubMed 16311592 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pAM-PAT-ProCDKA;1 GW 8400 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION binary DEST vector for plant expression.
ACCESSION .
VERSION .
KEYWORDS pAM-PAT-ProCDKA;1 GW
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8400)
AUTHORS Nowack MK, Grini PE, Jakoby MJ, Lafos M, Koncz C, Schnittger A
TITLE A positive signal from the fertilization of the egg cell sets off
endosperm proliferation in angiosperm embryogenesis.
JOURNAL Nat Genet. 2006 Jan;38(1):63-7. Epub 2005 Nov 27.
PUBMED 16311592
REFERENCE 2 (bases 1 to 8400)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8400
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 348..372
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
rep_origin 484..1193
/label=oriV
/note="incP origin of replication"
promoter 1266..1357
/gene="bla"
/label=AmpR promoter
CDS 1358..2218
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(1576..1595)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 2389..2977
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2878..2897
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
misc_feature 3289..3313
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
terminator 3407..3659
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
CDS complement(3679..4230)
/codon_start=1
/gene="Streptomyces hygroscopicus bar"
/product="phosphinothricin acetyltransferase"
/label=BlpR
/note="confers resistance to bialophos or phosphinothricin"
/translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
WQLDFSLPVPPRPVLPVTEI"
promoter complement(4272..4451)
/label=NOS promoter
/note="nopaline synthase promoter"
protein_bind 6673..6797
/gene="mutant version of attR"
/label=attR1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
promoter 6822..6852
/label=lac UV5 promoter
/note="E. coli lac promoter with an ""up"" mutation"
CDS 6906..7565
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind complement(6972..6991)
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
CDS 7907..8212
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind 8091..8110
/label=ccdB-fwd
/note="ccdB gene, forward primer"
protein_bind complement(8253..8377)
/gene="mutant version of attR"
/label=attR2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
ORIGIN
1 agcttgttga gaattcatcg cccgggactg ggttaacttc cgatcgattc tagactagtt
61 ctagagtccg caaaaatcac cagtctctct ctacaaatct atctctctct atttttctcc
121 agaataatgt gtgagtagtt cccagataag ggaattaggg ttcttatagg gtttcgctca
181 tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa
241 taaaatttct aattcctaaa accaaaatcc agtgaccggg cggccgccac cgcggtggag
301 ggggatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga caggatatat
361 tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg
421 tgaaaaggtt tatccgttcg tccatttgta tgtgtacatc accgacgagc aaggcaagac
481 cgagcgcctt tccgacgctc accgggctgg ttgccctcgc cgctgggctg gcggccgtct
541 atggccctgc aaacgcgcca gaaacgccgt cgaagccgtg tgcgagacac cggccgccgg
601 cgttgtggat acctcgcgga aaacttggcc ctcactgaca gatgaggggc ggacgttgac
661 acttgagggg ccgactcacc cggcgcggcg ttgacagatg aggggcaggc tcgatttcgg
721 ccggcgacgt ggagctggcc agcctcgcaa atcggcgaaa acgcctgatt ttacgcgagt
781 ttcccacaga tgatgtggac aagcctgggg ataagtgccc tgcggtattg acacttgagg
841 ggcgcgacta ctgacagatg aggggcgcga tccttgacac ttgaggggca gagtgctgac
901 agatgagggg cgcacctatt gacatttgag gggctgtcca caggcagaaa atccagcatt
961 tgcaagggtt tccgcccgtt tttcggccac cgctaacctg tcttttaacc tgcttttaaa
1021 ccaatattta taaaccttgt ttttaaccag ggctgcgccc tgtgcgcgtg accgcgcacg
1081 ccgaaggggg gtgccccccc ttctcgaacc ctcccggccc gctaacgcgg gcctcccatc
1141 cccccagggg ctgcgcccct cggccgcgaa cggcctcacc ccaaaaatgg cagcgctggc
1201 agtccttgcc attgccggga tcggggcagt aacgggatgg gcgatcagcc cgaaagctac
1261 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
1321 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
1381 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
1441 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
1501 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
1561 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
1621 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
1681 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
1741 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
1801 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
1861 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
1921 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
1981 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
2041 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
2101 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
2161 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
2221 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
2281 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
2341 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
2401 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
2461 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
2521 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
2581 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
2641 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
2701 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
2761 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
2821 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
2881 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
2941 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
3001 acggttcctg gccttttgct ggccttttgc tcacatggac tctagctaga ggatcacagg
3061 cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc atccgtgttt
3121 caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg
3181 ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc ctgtatcgag
3241 tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg gcaggatata
3301 ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga cgtttttaat
3361 gatcgaatac taacgtctct accagatatc agcttgcatg ccggtcgatc tagtaacata
3421 gatgacaccg cgcgcgataa tttatcctag tttgcgcgct atattttgtt ttctatcgcg
3481 tattaaatgt ataattgcgg gactctaatc ataaaaaccc atctcataaa taacgtcatg
3541 cattacatgt taattattac atgcttaacg taattcaaca gaaattatat gataatcatc
3601 gcaagaccgg caacaggatt caatcttaag aaactttatt gccaaatgtt tgaacgatct
3661 gcttgactct aggggtcatc agatttcggt gacgggcagg accggacggg gcggcaccgg
3721 caggctgaag tccagctgcc agaaacccac gtcatgccag ttcccgtgct tgaagccggc
3781 cgcccgcagc atgccgcggg gggcatatcc gagcgcctcg tgcatgcgca cgctcgggtc
3841 gttgggcagc ccgatgacag cgaccacgct cttgaagccc tgtgcctcca gggacttcag
3901 caggtgggtg tagagcgtgg agcccagtcc cgtccgctgg tggcgggggg agacgtacac
3961 ggtcgactcg gccgtccagt cgtaggcgtt gcgtgccttc cagggacccg cgtaggcgat
4021 gccggcgacc tcgccgtcca cctcggcgac gagccaggga tagcgctccc gcagacggac
4081 gaggtcgtcc gtccactcct gcggttcctg cggctcggta cggaagttga ccgtgcttgt
4141 ctcgatgtag tggttgacga tggtgcagac cgccggcatg tccgcctcgg tggcacggcg
4201 gatgtcggcc gggcgtcgtt ctgggctcat ggtagatccc ctcgatcgag ttgagagtga
4261 atatgagact ctaattggat accgagggga atttatggaa cgtcagtgga gcatttttga
4321 caagaaatat ttgctagctg atagtgacct taggcgactt ttgaacgcgc aataatggtt
4381 tctgacgtat gtgcttagct cattaaactc cagaaacccg cggctcagtg gctccttcaa
4441 cgttgcggtt ctgtcagttc caaacgtaaa acggcttgtc ccgcgtcatc ggcgggggtc
4501 ataacgtgac tcccttaatt ctccgctcat gatcagattg tcgtttcccg ccttcggttt
4561 gggcgcgccg ggaagataga agggaagaga gaggtagacg gaaagagaaa tggagaaaaa
4621 tagataaagg ggtaaacttg gaattaatat acaaaatata gaggatatca gttctttttc
4681 cttttgatgt tagtttctta attataaact ttttgttggt tagttttgcc aattccctta
4741 cttacaactg ataaccgtat agctctttta tcagaaaaaa ctttatatat ctaatctccc
4801 aaaatataca aatgtgagaa atatctgaaa ataaatcaat tctcaccaat tatatatatt
4861 tgattactta aaaatgttaa tatatggcag ttgcttgttc agagaataac ttaaaaaatt
4921 gatcacgaga tcggcttatg tttaatattt ttgaactctt gggataaaag ttttgaagat
4981 atatatatcg tagaaaagtt ttgtgttctt taattagtta caaaattttg agtttttttt
5041 ttttctttca taaagtatca gaatatcagg gtgatttatt gaatagttta ttttttctcg
5101 ttctactcac gttattcttg caagtattaa ttttggtttg tttataaaaa aagtatttgg
5161 aaattttatg tgagcaagtg cgaaataaac gaggattttg ggaagtattt ttttttttgt
5221 aaaatcggtt atattgaacg ttatactata tatattatta tataaatata actgctaaag
5281 actaaattta gatactaaaa tcagcattga tgaagatcca catgaataag actcgtctct
5341 taactcaaca tttaacatat gattttcata ttattgatca tttatctttg tgttggtcca
5401 aggtgccaga ctttattatt tacattaaat gttgagttgt ttactgacat attttttaat
5461 taaaacaact ttcatattat tgcccattta tattttggtt ggtcctccaa agacttacag
5521 gatacatatg tttctccatt ttcattttta tgatctaaaa cgtctttttc taacttagat
5581 tctaaatggt tacagcaaac cggacataca attttgcttg tcaaatggat cgttgagata
5641 gacaacaatc caatgtaaaa ccaaaactct tgatttataa ttggtctgca atgatttttt
5701 tttggttcat ataccatttt ataagatagc aaaccatttt catcataaaa gatatatttt
5761 tttatcattg aataccaatc aaacccggta taataaataa aattgtacca aattaatgcg
5821 gttaggtaaa ttcaatttat tggaaattaa agaagagaaa aaaaaaataa cgaacaaaat
5881 ttccttgaca caaaaaaaaa aaaagaagaa agaaaaaacc cttactctca attagtcaat
5941 ccccttccct agtcctcctc tcttcaagct tttcacagaa aaccaccacc cttctctctc
6001 tactgccttt ttaccacaga gaagagagag gatccgtcgg tgtgctagtc tcactgacac
6061 tacatccgat cgtcgccggt aagttgattt cgttactctc cgatcaattt cttttccgga
6121 ggaggaactt gttttccgtg cgaaattctg acttgaactt tgttgtcaat tcatcaaacg
6181 ctttatcttt tctccatctt caattatctt actgcttcgt cgctgatctc tacttgtttt
6241 cctccgtttt gcattttcag tggcgtttct tagtgtttca ctagatttag actggttttg
6301 cttgtgtgca ttttgtcatc agtgtttatg attgttcgct ttagctctat gtttctatgg
6361 atttgataat atatgagttc gctgctctgg attcagctgg cttgtttgat tgttcatttt
6421 cataattctg tccaaagaat caaatccaca gtagctcgga tctgagattg atttgtttct
6481 ttgcgtcttt gtgtagattt gactttaatt ttgagattgt tgtgtgctga tatacagctt
6541 aattaatata gcctctgatg tgttattatc gatgtgtcat tatcaatcta caggtgacat
6601 tttataagtg tggagtttac ttcagcttta ttattcagga attgctcgag aaagaggatc
6661 cacctgagga tcacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat
6721 atcaatatat taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa
6781 catatccagt cactatggcg gccgcattag gcaccccagg ctttacactt tatgcttccg
6841 gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga gctaaggaag
6901 ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta
6961 aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc
7021 tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct
7081 ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag
7141 acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa
7201 ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca
7261 tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta
7321 ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa
7381 acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc
7441 aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct
7501 tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg
7561 cgtaaacgcg tggatccggc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg
7621 atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggta
7681 tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc
7741 atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt
7801 ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt
7861 attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc agtttaaggt
7921 ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat
7981 tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa
8041 agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac
8101 caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca
8161 ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg
8221 ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt tgtgttttac
8281 agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat
8341 tttacgtttc tcgttcagct ttcttgtaca aagtggtgat cctccgttcc atgggctaga
//
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